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KMID : 0613820070170010051
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Journal of Life Science
2007 Volume.17 No. 1 p.51 ~ p.55
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Antioxidant Activities of Ulva lactuca Extracts with Different Solvents
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Li Huayue
Lee Sung-Mok
Lee Dong-Geun
Lee Jae-Hwa
Lee Sang-Hyeon
Ha Bae-Jin
Jang Jeong-Su
Kim Won-Suk
Ha Jong-Myung
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Abstract
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The fractions of Ulva lactuca were studied to verify the antioxidant activities. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with the solvents such as hexane, ethyl ether, ethyl acetate, butanol and H₂O. Furthermore, ethyl ether, ethyl acetate and aqueous fractions of U. lactuca were purified using HPLC. The antioxidant activities of purified samples from ethyl ether, ethyl acetate, and aqueous fractions were investigated using 1,1-diphenyl-2-picryl-hydrazil (DPPH). L-ascorbic acid, a positive control showed the highest DPPH radical scavenging activity. In addition, purified sample from aqueous fraction also showed relatively high activity. Purified sample from ethyl acetate fraction showed moderate activity, but purified sample from ethyl ether fraction showed the lowest activity. Dose dependent patterns were observed on all three samples tested. The lipid peroxidation inhibition activities of these three purified samples were also investigated. Purified sample from ethyl ether fraction of U. lactuca showed the highest activity and as strong activity as that of α-tocopherol, a positive control. These results suggest that U. lactuca may be a useful candidate for a natural antioxidant agent.
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KEYWORD
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Ulva lactuca, HPLC, antioxidant, DPPH, lipid peroxidation
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